BD-PE4916-RSPO3 (6F10) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RSPO3 (6F10) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4916

产品名称

RSPO3 (6F10) Mouse Monoclonal antibody

类别

常规抗体

基因名称

RSPO3

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 22-272 of human RSPO3 (NP_116173) produced in E.coli.

稀释度

WB 1:500, IHC 1:150

预测分子量

30.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development. [provided by RefSeq, Jul 2013]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-RSPO3 mouse monoclonal antibody. (BD-PE4916; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-RSPO3 mouse monoclonal antibody. (BD-PE4916; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RSPO3 mouse monoclonal antibody. (BD-PE4916; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RSPO3 mouse monoclonal antibody. (BD-PE4916; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RSPO3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RSPO3(Cat# BD-PE4916). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.