BD-PE4875-SPANXB1 (7T11) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SPANXB1 (7T11) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4875

产品名称

SPANXB1 (7T11) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SPANXB1

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human SPANXB1 (NP_115850) produced in E.coli.

稀释度

WB 1:500~2000, IHC 1:1000

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular family member contains an additional 18 nucleotides in its coding region compared to the other family members in the same gene cluster. This family member is also subject to gene copy number variation. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis. [provided by RefSeq, Apr 2014]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from MDA-MB231 cell line by using anti-SPANXB1 monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded Human gastric stromal tumor tissue using anti-SPANXB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE4875) (1:1000)

Immunohistochemical staining of paraffin-embedded Human testicle tissue within the normal limits using anti-SPANXB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE4875) (1:1000)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human spleen tissue within the normal limits using anti-SPANXB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE4875) (1:1000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPANXB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPANXB1 (1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.