Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PCDH7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE4874)

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PCDH7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCDH7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCDH7(Cat# BD-PE4874). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (50ug per lane) from 3 cell lines lysates by using anti-PCDH7 monoclonal antibody(BD-PE4874, 1:500)

Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-PCDH7 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (lane 1) , human PCDH7 plasmid (lane 2), mouse PCDH7 plasmid (lane 3), , using anti-PCDH7 antibody BD-PE4874 (1:500).

Anti-PCDH7 mouse monoclonal antibody (BD-PE4874) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PCDH7 .

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PCDH7 monoclonal antibody (1:200).