OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-WIBG mouse monoclonal antibody (BD-PE4854). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500

Immunofluorescent staining of HeLa cells using anti-WIBG mouse monoclonal antibody (BD-PE4854, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded liver cancer with mouse anti-WIBG 1:400 of 1mg/mL using HIER TEE pH9.0 . Expression of WIBG is cytoplasmic.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-WIBG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, BD-PE4854) (1:500)

Immunohistochemical staining of paraffin-embedded ovarian cancer with mouse anti-WIBG 1:400 of 1mg/mL using HIER TEE pH9.0 . Expression of WIBG is cytoplasmic.

Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-WIBG monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen). (1:500) Dilution: 1:500

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-WIBG mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, BD-PE4854) (1:500)