BD-PE4853-WIBG (6Z9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

WIBG (6Z9) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4853

产品名称

WIBG (6Z9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

WIBG

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human WIBG (NP_115721) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~500

预测分子量

22.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

WIBG (within bgcn homolog (Drosophila)) is a protein-coding gene. Diseases associated with WIBG include mucoepidermoid carcinoma, and tongue cancer. GO annotations related to this gene include ribosome binding and RNA binding.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-WIBG mouse monoclonal antibody (BD-PE4853). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Immunohistochemical staining of paraffin-embedded colon cancer with mouse anti-WIBG 1:400 of 1mg/mL using HIER TEE pH9.0 . Expression of WIBG is cytoplasmic.

Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-WIBG monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen). (1:500) Dilution: 1:500

Immunohistochemical staining of paraffin-embedded pancreas with mouse anti-WIBG 1:400 of 1mg/mL using HIER TEE pH9.0 . Expression of WIBG is cytoplasmic.

Immunohistochemical staining of paraffin-embedded thyroid with mouse anti-WIBG 1:400 of 1mg/mL using HIER TEE pH9.0 . Expression of WIBG is cytoplasmic.

Immunohistochemical staining of paraffin-embedded ovarian cancer with mouse anti-WIBG 1:400 of 1mg/mL using HIER TEE pH9.0 . Expression of WIBG is cytoplasmic.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

Immunohistochemical staining of paraffin-embedded kidney with mouse anti-WIBG 1:400 of 1mg/mL using HIER TEE pH9.0 . Expression of WIBG is cytoplasmic.

Immunofluorescent staining of HeLa cells using anti-WIBG mouse monoclonal antibody (BD-PE4853, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded breast carcinoma with mouse anti-WIBG 1:400 of 1mg/mL using HIER TEE pH9.0 . Expression of WIBG is cytoplasmic.

Immunohistochemical staining of paraffin-embedded normal/normal adjacent breast with mouse anti-WIBG 1:400 of 1mg/mL using HIER TEE pH9.0 . Expression of WIBG is cytoplasmic and some nuclear in normal/normal adjacent breast tissue.