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MARK4 (10H19) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE4779
产品名称
MARK4 (10H19) Mouse Monoclonal antibody
类别
常规抗体
基因名称
MARK4
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Human recombinant protein fragment corresponding to amino acids 390-467 of human MARK4(NP_113605) produced in E.coli.
稀释度
IHC 1:150
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene encodes a member of the microtubule affinity-regulating kinase family. These protein kinases phosphorylate microtubule-associated proteins and regulate the transition between stable and dynamic microtubules. The encoded protein is associated with the centrosome throughout mitosis and may be involved in cell cycle control. Expression of this gene is a potential marker for cancer, and the encoded protein may also play a role in Alzheimer's disease. Pseudogenes of this gene are located on both the short and long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-MARK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE4779) (1:150)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MARK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE4779) (1:150)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MARK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE4779) (1:150)

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-MARK4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE4779) (1:150)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MARK4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK4(Cat# BD-PE4779). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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