产品名称
TNKS1 Rabbit Polyclonal Antibody
基因名称
TNKS PARP5A PARPL TIN1 TINF1 TNKS1
蛋白名称
Tankyrase-1 (TANK1) (EC 2.4.2.30) (ADP-ribosyltransferase diphtheria toxin-like 5) (ARTD5) (Poly [ADP-ribose] polymerase 5A) (TNKS-1) (TRF1-interacting ankyrin-related ADP-ribose polymerase) (Tankyrase I)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
O95271
Human Swissprot Link
https://www.uniprot.org/uniprotkb/O95271/entry
Mouse Swissprot No.
Q6PFX9
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q6PFX9
免疫原
Synthesized peptide derived from part region of human protein
特异性
TNKS1 Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:May regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Has PARP activity and can modify TERF1, and thereby contribute to the regulation of telomere length.,PTM:ADP-ribosylated (-auto).,PTM:Phosphorylated on serine residues by MAPK kinases upon insulin stimulation.,similarity:Contains 1 PARP catalytic domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 15 ANK repeats.,subcellular location:Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1.,subunit:Oligomerizes and associates with TNKS2. Interacts with the cytoplasmic domain of LNPEP/Otase in SLC2A4/GLUT4-vesicles. Binds to the N-terminus of telomeric TERF1 via the ANK repeats. Found in a complex with POT1; TERF1 and TINF2.,tissue specificity:Ubiquitous; highest levels in testis.,
组织表达
Ubiquitous; highest levels in testis.
细胞定位
Cytoplasm . Golgi apparatus membrane ; Peripheral membrane protein . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nucleus, nuclear pore complex . Chromosome, telomere . Cytoplasm, cytoskeleton, spindle pole . Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles (PubMed:22864114). A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres (PubMed:10523501). During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1 (PubMed:12768206). Localizes to spindle poles at mitosis onset via interaction with NUMA1 (PubMed:12080061). .
功能
catalytic activity:NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.,function:May regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. Has PARP activity and can modify TERF1, and thereby contribute to the regulation of telomere length.,PTM:ADP-ribosylated (-auto).,PTM:Phosphorylated on serine residues by MAPK kinases upon insulin stimulation.,similarity:Contains 1 PARP catalytic domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 15 ANK repeats.,subcellular location:Associated with the Golgi and with juxtanuclear SLC2A4/GLUT4-vesicles. A minor proportion is also found at nuclear pore complexes and around the pericentriolar matrix of mitotic centromeres. During interphase, a small fraction of TNKS is found in the nucleus, associated with TERF1.,subunit:Oligomerizes and associates with TNKS2. Interacts with the cytoplasmic domain of LNPEP/Otase in SLC2A4/GLUT4-vesicles. Binds to the N-terminus of telomeric TERF1 via the ANK repeats. Found in a complex with POT1; TERF1 and TINF2.,tissue specificity:Ubiquitous; highest levels in testis.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
