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SERPINB1 (13K10) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE4741
产品名称
SERPINB1 (13K10) Mouse Monoclonal antibody
类别
常规抗体
基因名称
SERPINB1
推荐应用
WB
反应种属
Human,Dog,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Human recombinant protein fragment corresponding to amino acids 77-306 of human SERPINB1 (NP_109591) produced in E.coli.
稀释度
WB 1:500~2000, IHC 1:150, FCM 1:100
预测分子量
42.6kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2b
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINB1 antibody (BD-PE4741), and then analyzed by flow cytometry.

Flow cytometric Analysis of Jurkat cells, using anti-SERPINB1 antibody (BD-PE4741), (Red), compared to a nonspecific negative control antibody , (Blue).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4741)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4741)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4741)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4741)

Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SERPINB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4741)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4741)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4741)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4741)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SERPINB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4741)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINB1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINB1. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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