BD-PE4723-CD276 (18I1) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD276 (18I1) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4723

产品名称

CD276 (18I1) Mouse Monoclonal antibody

类别

常规抗体

基因名称

B7H3

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CD276 (NP_079516) produced in HEK293T cell.

稀释度

WB 1:500-1000, FCM 1:100

预测分子量

33.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011].

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric analysis of living 293T cells transfected with CD276 overexpression plasmid , Red)/empty vector (Blue) using anti-CD276 antibody (BD-PE4723). Cells incubated with a non-specific antibody (Green) were used as isotype control.（1：100）

Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CD276 monoclonal antibody. (1:500)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD276 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD276 antibody (Cat# BD-PE4723)(1:1000)

Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CD276 monoclonal antibody. (1:500)