OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PDCD1LG2 mouse monoclonal antibody (BD-PE4722). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDL2 (1:1k)

Flow cytometric analysis of living 293T cells transfected with PDL2 overexpression plasmid (Red)/empty vector (Blue) using anti-PDL2 antibody (BD-PE4722). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDCD1LG2 mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. BD-PE4722 was diluted 1:500 and detection was done with HRP secondary and DAB chromogen. Cytoplasmic and membraneous staining seen in tumor cells.

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PDCD1LG2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 2.5 min, BD-PE4722) (1:500)