BD-PE4713-WDR61 (9Y1) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

WDR61 (9Y1) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4713

产品名称

WDR61 (9Y1) Mouse Monoclonal antibody

类别

常规抗体

基因名称

WDR61

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 92-305 of human WDR61(NP_079510) produced in E.coli.

稀释度

WB 1:2000, IHC 1:150

预测分子量

33.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]). [supplied by OMIM, Mar 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-WDR61 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE4713)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-WDR61 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE4713)

Western blot analysis of extracts (10ug) from 5 different cell lines and 4 human tissue by using anti-WDR61 monoclonal antibody (1: Hela; 2: HepG2; 3: K562; 4: Mcf7; 5: SVT2; 6: Liver; 7: Testis; 8: Brain; 9: Uterus)at 1:200 dilution.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-WDR61 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE4713)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WDR61 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR61. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.