BD-PE4620-NDUFAF5 (17P14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

NDUFAF5 (17P14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4620

产品名称

NDUFAF5 (17P14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

NDUFAF5

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human NDUFAF5 (NP_077025) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:2000

预测分子量

38.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NDUFAF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE4620) (1:2000)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NDUFAF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE4620) (1:2000)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NDUFAF5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE4620) (1:2000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFAF5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFAF5 (Cat# BD-PE4620)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.