BD-PE4594-MRPS34 (5O9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MRPS34 (5O9) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4594

产品名称

MRPS34 (5O9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MRPS34

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human MRPS34(NP_076425) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~200

预测分子量

25.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternate splice variants of this gene have been described but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-MRPS34 mouse monoclonal antibody (BD-PE4594). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MRPS34mouse monoclonal antibody. (BD-PE4594; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human brain using anti-MRPS34 25 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE4594 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in.

Immunohistochemical staining of paraffin-embedded human breast normal adjacent using anti-MRPS34 25 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE4594 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in breast epithelia cells.

Immunohistochemical staining of paraffin-embedded human pituitary using anti-MRPS34 25 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE4594 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in breast epithelia cells.

Immunohistochemical staining of paraffin-embedded human liver normal adjacent using anti-MRPS34 25 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE4594 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and puntate cytoplasmic staining in hepatocyte cells

Immunohistochemical staining of paraffin-embedded human kidney normal adjacent using anti-MRPS34 25 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE4594 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in the normal kidney epithelia cells.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MRPS34 mouse monoclonal antibody. (BD-PE4594; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human thyroid cancer using anti-MRPS34 25 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE4594 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in tumor cells.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

Immunohistochemical staining of paraffin-embedded human lung cancer using anti-MRPS34 25 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE4594 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in tumor cells.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded human colon normal adjacent using anti-MRPS34 25 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE4594 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in the normal epithelia cells.

Immunohistochemical staining of paraffin-embedded human colon cancer using anti-MRPS34 25 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE4594 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in the tumor cells.

Immunofluorescent staining of HeLa cells using anti-MRPS34 mouse monoclonal antibody (BD-PE4594, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRPS34 mouse monoclonal antibody. (BD-PE4594; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)