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TRMT2A (5Z1) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE4529
产品名称
TRMT2A (5Z1) Mouse Monoclonal antibody
类别
常规抗体
基因名称
TRMT2A
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human TRMT2A(NP_073564) produced in HEK293T cell.
稀释度
WB 1:2000, IHC 1:150
预测分子量
68.5kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
The protein encoded by this gene is of unknown function. However, it is orthologous to the mouse Trmt2a gene and contains an RNA methyltransferase domain. Expression of this gene varies during the cell cycle, with aberrant expression being a possible biomarker in certain breast cancers. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TRMT2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE4529)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRMT2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE4529)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRMT2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRMT2A. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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