BD-PE4521-XRCC4 (12J9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

XRCC4 (12J9) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4521

产品名称

XRCC4 (12J9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

XRCC4

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full-length protein expressed in 293T cell transfected with human XRCC4 expression vector

稀释度

WB 1:2000, IHC 1:50

预测分子量

38.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4521, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4521, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4521, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4521, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4521, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4521, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4521, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4521, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-XRCC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4521, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4521, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4521, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4521, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-XRCC4mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4521, Dilution 1:50)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC4(Cat# BD-PE4521). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.