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MPP5 (9Y12) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE4516
产品名称
MPP5 (9Y12) Mouse Monoclonal antibody
类别
常规抗体
基因名称
MPP5
推荐应用
WB
反应种属
Human,Dog,Rat,Mouse
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human MPP5(NP_071919) produced in HEK293T cell.
稀释度
WB 1:500~2000, FCM 1:100
预测分子量
77.1kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2a
背景介绍
This gene encodes a member of the p55-like subfamily of the membrane-associated guanylate kinase (MAGUK) gene superfamily. The encoded protein participates in the polarization of differentiating cells, has been shown to regulate myelinating Schwann cells (PMID: 20237282), and is one of the components of the Crumbs complex in the retina. Mice which express lower levels of the orthologous protein have retinal degeneration and impaired vision (PMID: 22114289). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MPP5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Flow cytometric Analysis of Hela cells, using anti-MPP5 antibody (BD-PE4516), (Red), compared to a nonspecific negative control antibody, (Blue).

Flow cytometric Analysis of Jurkat cells, using anti-MPP5 antibody (BD-PE4516), (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MPP5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MPP5(Cat# BD-PE4516). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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