BD-PE4494-PDF (10G4) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PDF (10G4) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4494

产品名称

PDF (10G4) Mouse Monoclonal antibody

类别

常规抗体

基因名称

PDF

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human PDF (NP_071736) produced in E.coli.

稀释度

WB 1:500~2000, IF 1:100, FCM 1:100

预测分子量

19.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDF antibody (BD-PE4494), and then analyzed by flow cytometry.

Flow cytometric Analysis of Hela cells, using anti-PDF antibody (BD-PE4494), (Red), compared to a nonspecific negative control antibody , (Blue).

Anti-PDF mouse monoclonal antibody (BD-PE4494) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF.

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-PDF monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Flow cytometric Analysis of Jurkat cells, using anti-PDF antibody (BD-PE4494), (Red), compared to a nonspecific negative control antibody , (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF(Cat# BD-PE4494). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.