BD-PE4458-MCL1 (11T11) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MCL1 (11T11) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4458

产品名称

MCL1 (11T11) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MCL1

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human MCL1 (NP_068779) produced in HEK293T cell.

稀释度

WB 1:2000, IF 1:100, FCM 1:100

预测分子量

37.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

The protein encoded by this gene belongs to the Bcl-2 family. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. The longer gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene product (isoform 2) promotes apoptosis and is death-inducing.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric analysis of HEK293T cells (black), stained with anti-MCL1 antibody (BD-PE4458) (Blue) or Isotype IgG control antibody (Red).

Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-MCL1 monoclonal antibody at 1:200 dilution.

Immunofluorescent staining of HepG2 cells using anti-MCL1 mouse monoclonal antibody (BD-PE4458).

Anti-MCL1 mouse monoclonal antibody (BD-PE4458) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MCL1 .

Flow cytometric analysis of Hela cells, using anti-MCL1 antibody (BD-PE4458), (Red) compared to a nonspecific negative control antibody (Blue).

Flow cytometric analysis of Jurkat cells, using anti-MCL1 antibody (BD-PE4458), (Red) compared to a nonspecific negative control antibody (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MCL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MCL1(Cat# BD-PE4458). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.