BD-PE4453-Purified CD20 (MS4A1) (12M11) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

Purified CD20 (MS4A1) (12M11) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4453

产品名称

Purified CD20 (MS4A1) (12M11) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MS4A1

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human MS4A1 (NP_068769) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100

预测分子量

32.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. [provided by RefSeq, Jul

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-MS4A1 mouse monoclonal antibody (BD-PE4453). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MS4A1 mouse monoclonal antibody. (BD-PE4453; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunofluorescent staining of Jurkat cells using anti-CD20 mouse monoclonal antibody (BD-PE4453, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 4µm.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:250.

Immunohistochemical staining of paraffin-embedded human spleen using anti-CD20 mouse monoclonal antibody at 1:200 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE4453 requires heat-induced epitope retrieval withCitrate pH6.0 for 3minutes at110C in pressure chamber. The image shows the cells in the white pulp have strong cytoplasmic and membraneous staining.

Human tonsil screened with OriGene's Ms anti MS4A1 (CD20) (BD-PE4453 @ 1:800). HIER buffer TEE was used in pressure cooker. Secondary Polink 1 AP Mouse with out chromogen was use according to protocol data sheet. Cytoplasmic and membrane stain was visualized with GBI permanent red chromogen.

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MS4A1 mouse monoclonal antibody. (BD-PE4453; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)