BD-PE4445-RIC8A (RIC8) (6Z18) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RIC8A (RIC8) (6Z18) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4445

产品名称

RIC8A (RIC8) (6Z18) Mouse Monoclonal antibody

类别

常规抗体

基因名称

RIC8

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human RIC8A (NP_068751) produced in HEK293T cell.

稀释度

WB 1:1000, IHC 1:50, IF 1:100

预测分子量

59.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Guanine nucleotide exchange factor (GEF), which can activate some, but not all, G-alpha proteins. Able to activate GNAI1, GNAO1 and GNAQ, but not GNAS by exchanging bound GDP for free GTP. Involved in regulation of microtubule pulling forces during mitotic movement of chromosomes by stimulating G(i)-alpha protein, possibly leading to release G(i)-alpha-GTP and NuMA proteins from the NuMA-GPSM2-G(i)-alpha-GDP complex (By similarity). Also acts as an activator for G(q)-alpha (GNAQ) protein by enhancing the G(q)-coupled receptor-mediated ERK activation

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-RIC8A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4445, Dilution 1:50)

Immunofluorescent staining of HepG2 cells using anti-RIC8A mouse monoclonal antibody (BD-PE4445).

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-RIC8A monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Anti-RIC8A mouse monoclonal antibody (BD-PE4445) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RIC8A .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIC8A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIC8A(Cat# BD-PE4445). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.