BD-PE4420-FCGR2A (15K7) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

FCGR2A (15K7) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4420

产品名称

FCGR2A (15K7) Mouse Monoclonal antibody

类别

常规抗体

基因名称

FCGR2A

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~150

预测分子量

34.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-FCGR2A mouse monoclonal antibody (BD-PE4420). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-FCGR2A mouse monoclonal antibody. (BD-PE4420; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FCGR2A mouse monoclonal antibody. (BD-PE4420; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-FCGR2A mouse monoclonal antibody. (BD-PE4420; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A (1:500).

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid 9: Colon, 10: Spleen). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-FCGR2A mouse monoclonal antibody. (BD-PE4420; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-FCGR2A mouse monoclonal antibody. (BD-PE4420; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-FCGR2A monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FCGR2A mouse monoclonal antibody. (BD-PE4420; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)