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Ngn3 (Neurogenin3) (6N1) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE4358
产品名称
Ngn3 (Neurogenin3) (6N1) Mouse Monoclonal antibody
类别
常规抗体
基因名称
NEUROG3
推荐应用
WB
反应种属
Human
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Recombinant fragment expressed in E.coli corresponding to amino acids 1-214of human Ngn3
稀释度
WB: 1:500, IHC: 1:150
预测分子量
22.9kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2b
背景介绍
Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm (Gradwohl et al., 2000 [PubMed 10677506]).[
期货
2周
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody (BD-PE4358) at 1:150 dilution at 1:150 dilution.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEUROG3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUROG3. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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