Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4312)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NLN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4312)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4312)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4312)

Anti-NLN mouse monoclonal antibody (BD-PE4312) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NLN .

Flow cytometric Analysis of Jurkat cells, using anti-NLN antibody (BD-PE4312), (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NLN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NLN(Cat# BD-PE4312). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.