BD-PE4308-ABAT (17A17) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ABAT (17A17) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE4308

产品名称

ABAT (17A17) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ABAT

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 29-323 of human ABAT(NP_065737) produced in E.coli.

稀释度

IHC 1:1000

预测分子量

53.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500

Immunohistochemical staining of paraffin-embedded in human thyroid carcinoma using ABAT 80 mouse monoclonal antibody. Heat-induced epitope retrieval with 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 3min prior to BD-PE4308 diluted 1:1000 application. Detection with HRP enzyme and DAB chromogen was use to visualize result. Image shows strong cytoplasmic and membranous staining is present in the tumor cells.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, BD-PE4308) (1:1000)

Immunofluorescent staining of HepG2 cells using anti-ABAT mouse monoclonal antibody (BD-PE4308, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded human liver using ABAT 80, mouse monoclonal antibody at 1:1500 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. BD-PE4308 requires heat-induced epitope retrieval with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The image shows strong cytoplasmic and membranous staining of the hepatocytes no staining in the bile duct.

Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-ABAT monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen). (1:500) Dilution: 1:500

Immunohistochemical staining of paraffin-embedded human liver tissue using ABAT 80, mouse monoclonal antibody. Using heat-induced epitope retrieval with 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 3min prior in pressure chamber/cooker to BD-PE4308 diluted 1:1000 application. Detection with HRP enzyme and DAB chromogen was use to visualize result. Strong cytoplasmic and membranous staining is seen in the hepatocytes. Staining was not observed in the bile ducts of the liver.