BD-PE4307-ABAT (4V12) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ABAT (4V12) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4307

产品名称

ABAT (4V12) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ABAT

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 29-323 of human ABAT(NP_065737) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~200

预测分子量

53.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-ABAT mouse monoclonal antibody (BD-PE4307). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Immunohistochemical staining of paraffin-embedded carcinoma of human pancreas tissue using ABAT 78, mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE4307 was diluted 1:200 and detection shown with HRP enzyme and DAB chromogen. Images shows strong cytoplasmic and membranous staining is present in the tumor cells.

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-ABAT 78 mouse monoclonal antibody. HIER ACCEL buffer (pH8.7) at 110C for 10 min, BD-PE4307 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ABAT mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE4307) (1:200)

Immunohistochemical staining of paraffin-embedded human pancreas tissue using ABAT 78, mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE4307was diluted 1:200 and detection shown with HRP enzyme and DAB chromogen. Images shows strong cytoplasmic and membranous staining is present in the exocrine gladular cells.

Immunohistochemical staining of paraffin-embedded mouse liver tissue using anti-ABAT 78 mouse monoclonal antibody. HIER ACCEL buffer (pH8.7) at 110C for 10 min, BD-PE4307 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunofluorescent staining of HepG2 cells using anti-ABAT mouse monoclonal antibody (BD-PE4307, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded human liver using ABAT 78, mouse monoclonal antibody at 1:200 dilution of 1mg/mL using Polink2 Broad HRP DAB for detection. BD-PE4307 requires heat-induced epitope retrieval with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The image shows strong cytoplasmic and membranous staining of the hepatocytes no staining in the bile duct.

Immunohistochemical staining of paraffin-embedded carcinoma of human liver tissue using ABAT 78, mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE4307 was diluted 1:200 using HRP detection and DAB chromogen. Images shows strong cytoplasmic and membranous staining is present in the tumor cells.

Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-ABAT monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen). (1:500) Dilution: 1:500

Immunohistochemical staining of paraffin-embedded carcinoma of human kidney tissue using ABAT 78, mouse monoclonal antibody. Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE4307 was diluted 1:200 using HRP detection and DAB chromogen. Images shows strong cytoplasmic and membranous staining is present in the tumor cells.