BD-PE4300-RHOJ (16N2) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RHOJ (16N2) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4300

产品名称

RHOJ (16N2) Mouse Monoclonal antibody

类别

常规抗体

基因名称

RHOJ

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human RHOJ(NP_065714) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150, IF 1:100

预测分子量

23.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene encodes one of the many small GTP-binding proteins in the Rho family shown to be associated with focal adhesions in endothelial cells (PMID: 21148427, 22103495). The encoded protein is activated by vascular endothelial growth factor and may regulate angiogenesis. [provided by RefSeq, Dec 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RHOJ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE4300)

Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-RHOJ monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RHOJ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE4300)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RHOJ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE4300)

Anti-RHOJ mouse monoclonal antibody (BD-PE4300) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RHOJ .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RHOJ (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RHOJ. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.