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METTL3 (16O12) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE4218
产品名称
METTL3 (16O12) Mouse Monoclonal antibody
类别
常规抗体
基因名称
METTL3
推荐应用
WB
反应种属
Human,Rat,Mouse
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Human recombinant protein fragment corresponding to amino acids 1-284 of human METTL3 (NP_062826) produced in E.coli.
稀释度
WB 1:500~2000
预测分子量
64.3kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine. [provided by RefSeq, Jul 2008]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY METTL3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METTL3 (Cat# BD-PE4218)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug) from 6 different cell lines and 3 differenet tissue lysates by using anti-METTL3 monoclonal antibody (1:500).

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