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APOM (Apolipoprotein M) (17C6) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE4203
产品名称
APOM (Apolipoprotein M) (17C6) Mouse Monoclonal antibody
类别
常规抗体
基因名称
APOM
推荐应用
WB
反应种属
Human
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Recombinant protein expressed in E.coli corresponding to amino acids 23-188 of human APOM
稀释度
WB: 1:1000, IHC 1;50
预测分子量
21.1kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2b
背景介绍
The APOM is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Two transcript variants encoding two different isoforms have been found for this gene, but only one of them has been fully characterized
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded liver within the normal limits using anti-APOM (BD-PE4203) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).

Western blot analysis of extracts (10ug) from 1 cell line by using anti-APOM monoclonal antibody at 1:200 dilution.

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-APOM monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APOM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APOM(Cat# BD-PE4203). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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