BD-PE4198-DDX56 (7I4) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

DDX56 (7I4) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4198

产品名称

DDX56 (7I4) Mouse Monoclonal antibody

类别

常规抗体

基因名称

DDX56

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 323-547 of human DDX56 (NP_061955) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:2000, IHC 1:100~200

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene shows ATPase activity in the presence of polynucleotides and associates with nucleoplasmic 65S preribosomal particles. This gene may be involved in ribosome synthesis, most likely during assembly of the large 60S ribosomal subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-DDX56 mouse monoclonal antibody (BD-PE4198). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Western blot analysis of HCT116 cell lysate (35ug) by using anti-DDX56 monoclonal antibody.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen ). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Western blot of mouse tissue lysates (20ug) from Spleen. Primary antibody diluation: 1:500. Secondary antibody dilution: Mouse TrueBlot® Ultra (1:1000).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunofluorescent staining of HeLa cells using anti-DDX56 mouse monoclonal antibody (BD-PE4198, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-DDX56 mouse monoclonal antibody. (BD-PE4198; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)