HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP53 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP53 (Cat# BD-PE4192)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (NC) , human USP53 plasmid (hUSP53), mouse USP53 plasmid (mUSP53) using anti-USP53 antibody BD-PE4192 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (1:1000)