BD-PE4176-KRT20/CK20 (15W14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

KRT20/CK20 (15W14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4176

产品名称

KRT20/CK20 (15W14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

KRT20

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human KRT20 (NP_061883) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~200

预测分子量

48.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-KRT20 mouse monoclonal antibody (BD-PE4176). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KRT20 mouse monoclonal antibody. (BD-PE4176; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)

HEK293T were transfected with 55 different plasmids of with CK cDNA (1, 2, 4, 5, 6a, 6b, 6c, 7, 8, 9, 12, 13, 14, 15, 16, 17, 18 v1, 18 v2, 19, 20, 24, 25, 26, 27, 28, 31, 32, 33a, 33b, 34, 35, 36, 37, 38, 39, 40, 71, 72 v1, 72 v3, 73, 74, 75, 76, 77, 78, 79, 80 v1, 80 v2, 81, 82, 83, 84, 85, 86 and 222) for 48 hrs and lysed. Cell lysates (5 ug per lane) were separated by SDS-PAGE and blotted with KRT19 ab. KRT12, 19, 25, 26, 27, 28 and 39 were positive, while all others were negative (1:2000).

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: PC-12, 8: MDCK, 9: MCF7).

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.

Western blot of mouse tissue lysates (20ug) from Colon. Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-KRT20 mouse monoclonal antibody. (BD-PE4176; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)