Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KCTD5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-KCTD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4167, Dilution 1:50)

HEK293T cells transfected with either pCMV6-ENTRY KCTD5 (Red) or empty vector control plasmid (Blue) were immunostained with anti-KCTD5 mouse monoclonal (BD-PE4167), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-KCTD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4167, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-KCTD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4167, Dilution 1:50)

Immunofluorescent staining of HeLa cells using anti-KCTD5 mouse monoclonal antibody (BD-PE4167).

Anti-KCTD5 mouse monoclonal antibody (BD-PE4167) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KCTD5 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KCTD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KCTD5(Cat# BD-PE4167). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.