产品名称
ZP3 Rabbit Polyclonal Antibody
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/7784
Human Swissprot No.
P21754
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P21754/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/22788
Mouse Swissprot No.
P10761
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/P10761
Rat Gene Link
https://www.uniprot.org/uniprot/114639
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P97708
免疫原
Synthesized peptide derived from human ZP3 AA range: 210-260
特异性
This antibody detects endogenous levels of ZP3 at Human/Mouse/Rat
宿主
Polyclonal, Rabbit,IgG
背景介绍
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene. [provided by RefSeq, Jul 2008],
组织表达
Expressed in oocytes (at protein level).
细胞定位
[Processed zona pellucida sperm-binding protein 3]: Zona pellucida .; Cell membrane ; Single-pass type I membrane protein .
功能
domain:The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.,function:The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.,online information:Molecular chastity -Issue 93 of April 2008,PTM:N-glycosylated.,PTM:O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.,PTM:Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.,similarity:Belongs to the ZP domain family. ZPC subfamily.,similarity:Contains 1 ZP domain.,subunit:Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers.,tissue specificity:Oocytes.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
