产品名称
GPNMB Rabbit Polyclonal Antibody
基因名称
GPNMB HGFIN NMB UNQ1725/PRO9925
蛋白名称
Transmembrane glycoprotein NMB (Transmembrane glycoprotein HGFIN)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
Q14956
Human Swissprot Link
https://www.uniprot.org/uniprotkb/Q14956/entry
Mouse Swissprot No.
Q99P91
Mouse Swissprot Link
http://www.uniprot.org/uniprot/Q99P91
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q6P7C7
免疫原
Synthesized peptide derived from part region of human protein
特异性
GPNMB Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
glycoprotein nmb(GPNMB) Homo sapiens The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
组织表达
Widely expressed, but very low expression, if any, in the brain (PubMed:12609765, PubMed:16609006). Expressed in the epidermis with higher levels in melanocytes compared with keratinocytes and Langerhans cells (at protein level) (PubMed:29336782). Expressed in peripheral blood, but not bone marrow mononuclear cells (PubMed:12609765). Expressed in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level) (PubMed:16489096). May be overexpressed in various cancers, including melanoma and glioblastoma multiforme (PubMed:7814155, PubMed:16489096, PubMed:16609006).
细胞定位
Cell membrane; Single-pass type I membrane protein . Melanosome membrane ; Single-pass type I membrane protein . Early endosome membrane ; Single-pass type I membrane protein . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .
功能
developmental stage:Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines.,function:Could be a melanogenic enzyme.,similarity:Belongs to the Pmel-17/NMB family.,similarity:Contains 1 PKD domain.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,tissue specificity:Not restricted to the melanocytic lineage.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
