Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRR11 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (NC) , human PRR11 plasmid (hPRR11), mouse PRR11 plasmid (mPRR11) using anti-PRR11 antibody BD-PE4086 (1:100). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (1:1000)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PRR11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4086)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-PRR11 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE4086)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRR11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRR11. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.