Cn|En

现货抗体产品库

ECT2 (17G6) Mouse Monoclonal antibody

产品详情 相关文献 产品问答 相关产品

产品基本信息

产品货号
BD-PE4044
产品名称
ECT2 (17G6) Mouse Monoclonal antibody
类别
常规抗体
基因名称
ECT2
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Human recombinant protein fragment corresponding to amino acids 175-438 of human ECT2(NP_060568) produced in E.coli.
稀释度
WB 1:2000, IHC 1:150
预测分子量
99.9kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (10ug) from 1 cell line by using anti-ECT2 monoclonal antibody (1:200).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ECT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, BD-PE4044)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECT2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECT2(Cat# BD-PE4044). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

相关文献

产品问答

相关产品

免责声明| 法律支持| 联系方式

市场:027-65023363   行政/人事:027-62439686   邮箱:marketing@brainvta.com  

销售总监:张经理  18995532642  华东区:陈经理 18013970337   华南区:王经理 13100653525   华中/西区:杨经理 18186518905   华北区:张经理 18893721749

地址:中国武汉东湖高新区光谷七路128号中科开物产业园1号楼

Copyright © 武汉枢密脑科学技术有限公司. All RIGHTS RESERVED.
鄂ICP备2021009124号 DIGITAL BY VTHINK