BD-PE4010-GEMIN8 (7C9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

GEMIN8 (7C9) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE4010

产品名称

GEMIN8 (7C9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

GEMIN8

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human GEMIN8 (NP_060326) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~400

预测分子量

28.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is part of the SMN complex, which is necessary for spliceosomal snRNP assembly in the cytoplasm and pre-mRNA splicing in the nucleus. The encoded protein binds to both SMN1 and the GEMIN6/GEMIN7 heterodimer, mediating their interaction. This protein is found in nuclear Gemini of Cajal bodies (gems) and in the cytoplasm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-GEMIN8 mouse monoclonal antibody (BD-PE4010). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-GEMIN8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, BD-PE4010) (1:400)

Immunohistochemical staining of paraffin-embedded human pancreas using anti-GEMIN8 85 mouse monoclonal antibody at 1:400 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. BD-PE4010 requires HIER with with Accel 3in1 EDTA solution ph8.7 at 110°C for 3min using pressure chamber/cooker. The shows strong membraneous and cytoplasmic staining in exocrin cells.

Immunohistochemical staining of paraffin-embedded human kidney using anti-GEMIN8 85 mouse monoclonal antibody at 1:400 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. BD-PE4010 requires HIER with with Accel 3in1 EDTA solution ph8.7 at 110°C for 3min using pressure chamber/cooker. The tubule epithelia cells shows strong membraneous and cytoplasmic staining.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-GEMIN8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, BD-PE4010) (1:400)

Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-GEMIN8 monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen). (1:500) Dilution: 1:500

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-GEMIN8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110°C for 10min, BD-PE4010) (1:400)