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HAO1 (8G8) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE3953
产品名称
HAO1 (8G8) Mouse Monoclonal antibody
类别
常规抗体
基因名称
HAO1
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human HAO1 (NP_060015) produced in HEK293T cell.
稀释度
WB: 1:500-1:2000, IF: 1:100, FCM: 1:50-1:100
预测分子量
40.7kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq]. COMPLETENESS: full length.
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HAO1 antibody (BD-PE3953), and then analyzed by flow cytometry at 1:100

Anti-HAO1 mouse monoclonal antibody (BD-PE3953) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HAO1 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAO1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAO1(Cat# BD-PE3953). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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