BD-PE3944-CYTH2 (14W10) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CYTH2 (14W10) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3944

产品名称

CYTH2 (14W10) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CYTH2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CYTH2 (NP_059431) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150

预测分子量

46.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The encoded protein exhibits GEP activity in vitro with ARF1, ARF3, and ARF6 and is 83% homologous to CYTH1. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CYTH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3944) (1:150)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CYTH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3944) (1:150)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CYTH2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3944) (1:150)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYTH2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYTH2 (Cat# BD-PE3944)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.