BD-PE3932-PAX5 (10Z6) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PAX5 (10Z6) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3932

产品名称

PAX5 (10Z6) Mouse Monoclonal antibody

类别

常规抗体

基因名称

PAX5

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human PAX5 (NP_057953) produced in E.coli.

稀释度

WB 1:2000, IHC 1:150

预测分子量

42kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. This gene encodes the B-cell lineage specific activator protein that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE3932)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE3932) (1:200)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE3932)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE3932) (1:200)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE3932)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE3932) (1:200)

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PAX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE3932)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAX5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAX5(Cat# BD-PE3932). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.