BD-PE3920-SIRT6 (12M7) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SIRT6 (12M7) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE3920

产品名称

SIRT6 (12M7) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SIRT6

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 88-352 of human SIRT6(NP_057623) produced in E.coli.

稀释度

WB 1:500~2000, IF 1:100

预测分子量

38.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. Alternative splicing results in multiple transcript variants.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SIRT6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

Anti-SIRT6 mouse monoclonal antibody (BD-PE3920) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SIRT6 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SIRT6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SIRT6(Cat# BD-PE3920). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.