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LCMT1 (2K14) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE3888
产品名称
LCMT1 (2K14) Mouse Monoclonal antibody
类别
常规抗体
基因名称
LCMT1
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human LCMT1(NP_057393) produced in HEK293T cell.
稀释度
WB 1:2000, IHC 1:150, IF 1:100, FCM 1:100
预测分子量
38.2kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2b
背景介绍
LCMT1 catalyzes the methylation of the carboxyl group of the C-terminal leucine residue (leu309) of the catalytic subunit of protein phosphatase-2A (PPP2CA; MIM 176915) (De Baere et al., 1999 [PubMed 10600115]). [supplied by OMIM, Mar 2008]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LCMT1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LCMT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3888)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-LCMT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3888)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-LCMT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3888)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LCMT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3888)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LCMT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3888)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LCMT1 antibody (BD-PE3888), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LCMT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3888)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LCMT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3888)

Anti-LCMT1 mouse monoclonal antibody (BD-PE3888) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LCMT1 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LCMT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LCMT1. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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