产品名称
RAP1A Rabbit Polyclonal Antibody
蛋白名称
Ras-related protein Rap-1A (C21KG) (G-22K) (GTP-binding protein smg p21A) (Ras-related protein Krev-1)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P62834
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P62834/entry
Mouse Swissprot No.
P62835
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P62835
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P62836
免疫原
Synthesized peptide derived from part region of human protein
特异性
RAP1A Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 ELISA 1:5000-20000
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a member of the Ras family of small GTPases. The encoded protein undergoes a change in conformational state and activity, depending on whether it is bound to GTP or GDP. This protein is activated by several types of guanine nucleotide exchange factors (GEFs), and inactivated by two groups of GTPase-activating proteins (GAPs). The activation status of the encoded protein is therefore affected by the balance of intracellular levels of GEFs and GAPs. The encoded protein regulates signaling pathways that affect cell proliferation and adhesion, and may play a role in tumor malignancy. Pseudogenes of this gene have been defined on chromosomes 14 and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014],
组织表达
Brain,Platelet,Skin,Uterus,
细胞定位
Cell membrane ; Lipid-anchor . Cytoplasm . Cytoplasm, perinuclear region . Cell junction . Early endosome . Recruited from early endosome to late endosome compartment after nerve growth factor (NGF) stimulation. Localized with RAPGEF2 at cell-cell junctions (By similarity). Colocalized with RAPGEF2 in the perinuclear region. .
信号通路
MAPK_ERK_Growth;MAPK_G_Protein;Chemokine;Focal adhesion;Leukocyte transendothelial migration;Long-term potentiation;Neurotrophin;Renal cell carcinoma;
功能
enzyme regulation:Activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner, and GFR.,function:Induces morphological reversion of a cell line transformed by a Ras oncogene. Counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner.,similarity:Belongs to the small GTPase superfamily. Ras family.,subunit:In its GTP-bound form interacts with PLCE1 and RADIL. Interacts with SGSM1, SGSM2 and SGSM3.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
