BD-PE3868-LEF1 (12I9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

LEF1 (12I9) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3868

产品名称

LEF1 (12I9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

LEF1

推荐应用

反应种属

Human,Rat,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human LEF1 (NP_057353) produced in HEK293T cell.

稀释度

WB 1:500, IHC 1:500, IF 1:100

预测分子量

44kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-LEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3868) (1:500)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3868) (1:500)

Western blot analysis of extracts (35ug) from 2 different tissues by using anti-LEF1 monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LEF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LEF1 (Cat# BD-PE3868)(1:500). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug) from Colo205 cells by using anti-LEF1 monoclonal antibody (1:500).