BD-PE3860-SNX9 (7V18) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SNX9 (7V18) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3860

产品名称

SNX9 (7V18) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SNX9

推荐应用

反应种属

Human,Monkey,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human SNX9(NP_057308) produced in HEK293T cell.

稀释度

WB 1:1000~2000, IF 1:100, FCM 1:100

预测分子量

66.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but does contain a SH3 domain near its N-terminus. This protein interacts with the cytoplasmic domains of the precursor but not the processed forms of a disintegrin and metalloprotease domain 9 and 15. This protein binds the beta-appendage domain of adaptor protein 2 and may function to assist adaptor protein 2 in its role at the plasma membrane. This protein interacts with activated Cdc42-associated kinase-2 to regulate the degradation of epidermal growth factor receptor protein. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SNX9 monoclonal antibody.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNX9 antibody (BD-PE3860), and then analyzed by flow cytometry.

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SNX9 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Anti-SNX9 mouse monoclonal antibody (BD-PE3860) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNX9.

Flow cytometric Analysis of Jurkat cells, using anti-SNX9 antibody (BD-PE3860), (Red), compared to a nonspecific negative control antibody , (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNX9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNX9. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.