Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:250

Immunofluorescent staining of HeLa cells using anti-C14orf166 mouse monoclonal antibody (BD-PE3803, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-C14ORF166 82 mouse monoclonal antibody at 1:600 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE3803 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber. The image shows the tumor cells have strong nuclear and intermediate levels of cytoplasmic staining.

Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-C14orf166 monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen). (1:500) Dilution: 1:500

Immunohistochemical staining of paraffin-embedded human colon cancer using anti-C14ORF166 82 mouse monoclonal antibody at 1:600 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE3803 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber. The image shows the tumor cells have strong nuclear and weak cytoplasmic staining.