BD-PE3778-MRPS7 (17G8) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MRPS7 (17G8) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3778

产品名称

MRPS7 (17G8) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MRPS7

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 38-242 of human MRPS7 (NP_057055) produced in E.coli.

稀释度

WB 1:2000, IHC 1:200

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. In the prokaryotic ribosome, the comparable protein is thought to play an essential role in organizing the 3' domain of the 16 S rRNA in the vicinity of the P- and A-sites. Pseudogenes corresponding to this gene are found on chromosomes 8p and 12p. [provided by RefSeq, Jul

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-MRPS7 mouse monoclonal antibody. (BD-PE3778; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunofluorescent staining of MCF-7 cells using anti-MRPS7 mouse monoclonal antibody (BD-PE3778, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRPS7 mouse monoclonal antibody. (BD-PE3778; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-MRPS7 mouse monoclonal antibody. (BD-PE3778; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-MRPS7 mouse monoclonal antibody. (BD-PE3778; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Western blot analysis of HCT116 cell lysate (35ug) by using anti-MRPS7 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded human normal brain using anti-MRPS7 56 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE3778 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C.

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-MRPS7 mouse monoclonal antibody. (BD-PE3778; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human composit of placenta using anti-MRPS7 56 mouse monoclonal antibody at 1:200 dilution 1mg/mL and detection with Polink2 Broad HRP DAB. BD-PE3778 requires heat-induced epitope retrieval with citrate pH6.0 in a presure cooker for 3 minutes at 110C. The image shows membranous and cytoplasmic staining in trophoblast cells.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MRPS7 mouse monoclonal antibody. (BD-PE3778; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-MRPS7 mouse monoclonal antibody. (BD-PE3778; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MRPS7 mouse monoclonal antibody. (BD-PE3778; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-MRPS7 mouse monoclonal antibody. (BD-PE3778; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRPS7 mouse monoclonal antibody. (BD-PE3778; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min)