BD-PE3559-PPP1R15A (GADD34) (19S11) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PPP1R15A (GADD34) (19S11) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3559

产品名称

PPP1R15A (GADD34) (19S11) Mouse Monoclonal antibody

类别

常规抗体

基因名称

GADD34

推荐应用

反应种属

Human,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human PPP1R15A(NP_055145) produced in HEK293T cell.

稀释度

WB 1:500~2000, IF 1:100, FCM 1:100

预测分子量

73.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The induction of this gene by ionizing radiation occurs in certain cell lines regardless of p53 status, and its protein response is correlated with apoptosis following ionizing radiation. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (NC) , human GADD34 plasmid (hGADD34), mouse GADD34 plasmid (mGADD34) using anti-GADD34 antibody BD-PE3559(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (1:1000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP1R15A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP1R15A. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPP1R15A antibody (BD-PE3559), and then analyzed by flow cytometry.

Flow cytometric Analysis of Hela cells, using anti-PPP1R15A antibody (BD-PE3559), (Red), compared to a nonspecific negative control antibody, (Blue).

Anti-PPP1R15A mouse monoclonal antibody (BD-PE3559) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPP1R15A .

Flow cytometric Analysis of Jurkat cells, using anti-PPP1R15A antibody (BD-PE3559), (Red), compared to a nonspecific negative control antibody, (Blue).

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPP1R15A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).