产品名称
MAFG Rabbit Polyclonal Antibody
蛋白名称
Transcription factor MafG (V-maf musculoaponeurotic fibrosarcoma oncogene homolog G) (hMAF)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
O15525
Human Swissprot Link
https://www.uniprot.org/uniprotkb/O15525/entry
Mouse Swissprot No.
O54790
Mouse Swissprot Link
http://www.uniprot.org/uniprot/O54790
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941Q76MX4
免疫原
Synthesized peptide derived from part region of human protein
特异性
MAFG Polyclonal Antibody detects endogenous levels of protein.
宿主
Polyclonal, Rabbit,IgG
背景介绍
Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM, Mar 2010],
组织表达
Highly expressed in skeletal muscle. Also expressed in heart and brain.
功能
function:Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF-E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H(+).,PTM:Acetylated in erythroid cells by CREB-binding protein (CBP). Acetylation augments the DNA-binding activity of NFE2, but has no effect on binding NFE2.,PTM:Sumoylation at Lys-14 is required for active transcriptional repression.,similarity:Belongs to the bZIP family. Maf subfamily.,similarity:Contains 1 bZIP domain.,subunit:Homodimer or heterodimer. Homodimerization leads to transcriptional repression. Forms high affinity heterodimers with members of the CNC-bZIP family such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3. Interacts with NFE2; the interaction results in tranactivation activation. Interacts with CREBBP; the interaction leads to acetylation of the basic region of MAFG and stimulation of NFE2 transcriptional activity through increased DNA binding.,tissue specificity:Highly expressed in skeletal muscle. Also expressed in heart and brain.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
