BD-PE3505-CD5 (14G8) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD5 (14G8) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3505

产品名称

CD5 (14G8) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CD5

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.

稀释度

FCM 1:50

预测分子量

54.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD5 antibody (BD-PE3505), and then living cells were analyzed by flow cytometry.

Flow cytometric analysis of living CCRF-CEM cells, using anti-CD5 antibody (BD-PE3505), (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:100).

Flow cytometric analysis of living 50ng/ml IL2-stimulated Jurkat cells, using anti-CD5 antibody (BD-PE3505), (red), compared to an IgG isotype control, (green), and negative control (PBS), (blue) (1:100).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5. (1:5. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.